جستجوی آنتی بادیهای ضد آنتی ژنهای گروه یک HLA در سرم زنان باردار و چگونگی تغییرات تیتر آن در دوران بارداری

          Determination of HLA antigens has an extensive application in paternity,    Histocompatibility testing, and disease suseptibility identification. A vast    arrey of different HLA antisera are required for HLA typing. Such antisera can    be obtained from pregnant women, transfused patients, immunized animals    or  hybridoma cells, but still the sera of pregnant women is one of the best    sources for producing of HLA antisera. The pregnant women usually form    antibodies to the foreign HLA antigens of the fetus inherited from the father.     Due to the genetic variations among different races and the very high price    of these antibodies it is preferable to obtain these antisera from locally    pregnant women.           In this study, sera of 40 primiparous and 65 multiparous pregnant Iranian    women were screened monthly (for 3 times) againest their husband’s pure    lymphocytes, using the microlymphocytotoxicity method of NIH.           Among the primiparous 2 cases (5%) and among the multiparous    women 9 cases (13.8%) had HLA antibodies in their sera. The titer and score    of antibody were determined monthly in positive samples. The antibodies    had the highest level in the 6th month of pregnancy.           We concluded that the best source and the time to obtain HLA antisera    are multiparous women in the 6th month of pregnancy